Use of whole-body cryosectioning and desorption electrosprayionization mass spectrometry imaging to visualize alkaloiddistribution in poison frogs

Authors

Adriana M. Jeckel, University of Sao Paulo, Brazil
Kunihiro Matsumura, Osaka City University
Keisuke Nishikawa, Osaka City University
Yoshiki Morimoto, Osaka City University
Ralph Saporito, John Carroll UniversityFollow
Taran Grant, University of Sao Paulo, Brazil
Demian R. Ifa, York University

Document Type

Article

Publication Date

2020

Publication Title

Journal of mass spectrometry

Abstract

Ambient mass spectrometry is useful for analyzing compounds that would beaffected by other chemical procedures. Poison frogs are known to sequester alkaloidsfrom their diet, but the sequestration pathway is unknown. Here, we describemethods for whole-body cryosectioning of frogs and use desorption electrosprayionization mass spectrometry imaging (DESI-MSI) to map the orally administeredalkaloid histrionicotoxin 235A in a whole-body section of the poison frog Den-drobates tinctorius. Our results show that whole-body cryosectioning coupled withhistochemical staining and DESI-MSI is an effective technique to visualize alkaloiddistribution and help elucidate the mechanisms involved in alkaloid sequestration inpoison frogs.

Recommended Citation

Jeckel, Adriana M.; Matsumura, Kunihiro; Nishikawa, Keisuke; Morimoto, Yoshiki; Saporito, Ralph; Grant, Taran; and Ifa, Demian R., "Use of whole-body cryosectioning and desorption electrosprayionization mass spectrometry imaging to visualize alkaloiddistribution in poison frogs" (2020). 2020 Faculty Bibliography. 55.
https://collected.jcu.edu/fac_bib_2020/55

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