Date of Award
Dr. Yuh-Cherng Chai
The purpose of this experiment was to analyze chicken breast fractions through affinity chromatography for protein modifications by glutathione disulfide (GSSG). The fractions were incubated with different concentrations of GSSG to determine potential modified proteins. There were proteins modified with GSSG at 33 kDa in fraction I, at 16 kDa, 36 kDa, and 44kDa in fraction II, and at 36 kDa in fraction III for the concentration dependent reactions. In the time dependent reaction for fraction II, there were two major proteins at 65 kDa and 176 kDa that were modified by GSSG. Lastly, we suggest that the major modified protein in fraction III is glyceraldehyde 3- phosphate dehydrogenase (GAPDH), which has a molecular weight of about 36 kDa.
Fransisco, Isabella, "S-glutathionylation of Chicken Breast Extract" (2022). Senior Honors Projects. 138.
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