Document Type


Publication Date

Spring 2016


This project utilized gas chromatography – mass spectrometry (GC-MS) to analyze the lipid peroxidation products of linoleic acid, an essential ω-6 fatty acid, and its reaction with oxygen. Optimum reaction conditions were identified by testing various combinations of variables: buffer, pH, oxidant, and time. With treatment of optimum conditions, several products were identified including, 2,4-decadienal, 4-hydroxy-2-nonenal (HNE), and 4-oxo-2-nonenal (ONE). The mechanisms behind the formation of these products have been proposed by several researchers. It was identified that varying oxidant concentration, pH, or time greatly impacted the obtained concentrations of final oxidation products. Additionally, beta-carotene and Vitamin E were tested as antioxidant assays to assess their effectiveness in preventing oxidation. Tested assays were successful, with betacarotene reducing the concentration of oxidation product the most.

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